Электрофизиологические методы

Электрофизиологические методы дают возможность регистрировать только те процессы, которые происходят на постсинаптической мембране, а активность пресинапти- ческих аксонных окончаний оценивается этими методами лишь косвенно, по действию высвобождающегося из них ацетилхолина.

Между 1959 и 1964 гг. В. Уиттэкер в Кембриджском университете и Э. де Робертис в Университете Буэнос-Айреса вместе со своими коллегами предприняли первые шаги в изучении нервных окончаний прямыми методами. Они показали, что при гомогенизации мозговой ткани грызунов нервные окончания отрываются от аксонов и как бы запаиваются, образуя так называемые синаптосомы. Их можно затем отделить от остальных фрагментов ткани путем центрифугирования в градиенте плотности: в результате центрифугирования синаптосомы собираются в зоне с определенной плотностью. Таким образом, одна из важнейших частей нейрона может быть выделена и проанализирована независимо от остальных компонентов мозга.

Уиттэкер и де Робертис (независимо друг от друга) осуществили и следующий шаг. Они обнаружили, что можно разрушить внешнюю мембрану синаптосом и выделить имеющиеся внутри везикулы. Везикулы, как и ожидалось, действительно содержали ацетилхолин. Но оказалось, что не весь ацетилхолин синаптосом упакован в везикулы — часть его находится непосредственно в цитоплазме. Р. Марчбэнкс из Кембриджского университета тогда предположил, что этот цитоплазматический ацетилхолин также играет важную функциональную роль в проведении нервного импульса.

 

 

 

14 Comments

  1. I just want to tell you that I am all new to blogs and really loved your web page. Almost certainly I’m want to bookmark your blog post . You amazingly come with amazing writings. Kudos for sharing your web page.

  2. tiul:

    Heya i’m for the first time here. I came across this board and I find It really useful & it helped me out much. I hope to give something back and aid others like you aided me.

  3. Useful info. Fortunate me I found your website by accident, and I’m surprised why this accident did not took place in advance! I bookmarked it.

  4. Very efficiently written article. It will be valuable to anybody who employess it, as well as myself. Keep up the good work – i will definitely read more posts.

  5. I like the helpful info you provide in your articles. I’ll bookmark your blog and check again here regularly. I’m quite certain I will learn many new stuff right here! Best of luck for the next!

  6. You could certainly see your skills in the work you write. The world hopes for more passionate writers such as you who aren’t afraid to say how they believe. At all times go after your heart. «Everyone has his day and some days last longer than others.» by Sir Winston Leonard Spenser Churchill.

  7. Hello, i think that i saw you visited my web site thus i came to “return the favor”.I am trying to find things to enhance my site!I suppose its ok to use a few of your ideas!!

  8. tiul:

    Hi there! Someone in my Myspace group shared this website with us so I came to give it a look. I’m definitely loving the information. I’m book-marking and will be tweeting this to my followers! Excellent blog and fantastic design.

  9. Amazon announced brand new Kindle Fire HDX 7-inch and 8.9-inch tablets somewhat quietly late last week. According to a June. 25 ABC News report, this new tablet from Amazon could actually be an iPad monster. That could certainly offer consumers the opportunity for plenty of discounted prices and some sales on tablets come this holiday shopping season. They begin shipping on March. 18, 2013.It yields a lot harder, not really impossible, for international (as from Nigeria or Russia) or local scam artists to pull a first one done to by using fake profiles and stolen photographs.You really on important to last stage within the process – launching and promotion. Along with this step, your online site’s focus is online presence. Individuals that a web site without visitors and isn’t ranked with the search engines is totally useless. That’s where SEO kinds the advertisements will are presented in. Social Media and other stuff are included as well in method.It’s sure fun again now end up being «Cured» of «Incurable» MS (Multiple Sclerosis). Yeah, my libido is better also. LOL I sure don’t miss the wheelchair that my former caregiver wanted me to stay in, learn.For those looking to maximise their odds of success a great online video chat, Sweet webcam provides you many associated with communicating online, through text chat, voice, video perhaps a involving all three. The first thing it is advisable to do when seeking dating partners on the web is to join a. You are basically click clear of watching and chatting at absolutely no cost with your favorite webcam lovely women.Anastasia-International may be the industry leader with over 36,000,000 unique yearly users (this number is constantly rising). Approximately 1000 dating agencies a lot of major cities of ad units USSR work with them.Make associated with all characteristics on the internet dating web portal that help critiquing contacts; nearly all have bio pages on all contributors. Many have live free sex rooms. If you’ve any specific questions, suggestions, or concerns you can contact the site’s host or moderator.So that’s the reason why on the main page there is not any direct mention of a channel, since good news broadcast and both stations are of the same ownership. You will find the name of Valley News Live.One thing you will arrived at admire is basically that you simply will learn the mistakes that you could have been making. That’s the reason girls have always desired to move on, leave behind you, when just do not manage current them the close pleasure they so badly yearn to have.Was the Hustler pictorial her farewell to the photo-model enterprise? «Absolutely not! I’ll still be modelling, especially photography. I’m always traveling throughout the states and Canada for modeling your job. It’s good for the internet sites. It’s good content,» Gray said.

  10. I have read several just right stuff here. Certainly worth bookmarking for revisiting. I wonder how much effort you place to create the sort of excellent informative website.

  11. A person essentially lend a hand to make significantly articles I might state. This is the first time I frequented your website page and to this point? I surprised with the research you made to create this particular put up amazing. Wonderful job!

  12. I have read some just right stuff here. Definitely value bookmarking for revisiting. I wonder how so much attempt you place to make the sort of fantastic informative site.

  13. Hey there, You have done a fantastic job. I will definitely digg it and personally suggest to my friends. I’m confident they’ll be benefited from this website.

  14. Thanks for the sensible critique. Me & my neighbor were just preparing to do some research about this. We got a grab a book from our area library but I think I learned more from this post. I am very glad to see such magnificent information being shared freely out there.

Оставить коментарий