Повседневный инструмент

Моноклональные антитела стали повседневным инструментом для обнаружения, распознавания и выделения молекул из сложных смесей и из тканей. Их использование в медицине весьма многообещающе: они могут служить для разрушения злокачественных образований. Есть надежда, что удастся получить моноклональные антитела, которые будут взаимодействовать только с антигеном поверхности раковых клеток определенного вида. Если такие антитела соединить с каким- либо «противораковым» агентом, то получится иммунотоксин, нацеленный на конкретные опухолевые клетки и способный убить их, не причинив вреда нормальным клеткам.

НОБЕЛЕВСКУЮ премию по химии в 1984    г. получил Р. Брюс Мерри- филл за «простую и гениальную» идею автоматического синтеза белков, которая открывает совершенно новые возможности в области химии белков и пептидов, а также нуклеиновых кислот.

В конце 50-х годов Меррифилд начал разрабатывать способ последовательной сборки аминокислот в пептиды. Пептид — это как бы упрошенный вариант белка; пептиды, как и белки, представляют собой цепочки аминокислот, но гораздо более короткие. Метод Меррнфилда позволял осуществить автоматический синтез длинного белка. Первым полученным таким путем белком был инсулин, синтезированный Меррнфилдом и его коллегами в 1965 г. По методу Меррнфилда первая аминокислота связывается ковалентно с гранулой полистирола; остальные аминокислотные звенья присоединяются по одному в нужной последовательности путем стандартной реакции, которую можно авгомапнировать. По окончании синтеза образовавшаяся полипептидная цепь легко отделяется от носителя. Эта простая идея имела далеко идущие последствия. Появилась возможность исследовать, как зависят биологические функции белков (в том числе гормонов, ферментов и антител) от их структуры. Такого рода исследования важны потому, что находят практическое применение в медицине.

Предложенный Меррифнлдом способ синтеза белковых соединений оказался полезным при разработке метода моноклональных антител. На твердофазных носителях синтезируют пептидные антигены, которые в свою очередь нужны для получения клонов гибридных клеток, продуцирующих в большом количестве антитела к этим антигенам.

 

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