Высокая степень специфичности

Такая высокая степень специфичности вместе с большим числом гипотетических молекулярных меток остановила нас. Мы сочли, что рассматриваемая стадия развития с ее сотней взаимодействующих нейронов уже слишком сложна для точного клеточного и молекулярного анализа, и решили рассмотреть более ранние стадии. МЫ выбрали период между шестыми и седьмыми сутками эмбрионального развития, когда первые семь конусов роста в этой области эмбриона в результате специфичных взаимодействий друг с другом формируют первые три продольных аксонных пучка. Один пучок состоит из единственного аксона, принадлежащего нейрону vMP2, другой включает аксоны нейронов А/Р 1, (IMP2 и рСС, третий — аксоны клеток U, U2 и аСС. Совместно с С. дю Лаком мы исследовали выбор, проделываемый конусами роста нейронов рСС и аСС, которые являются сестринскими потомками одной ганглионарной материнской клетки.

Поверхности клеток рСС и аСС должны быть помечены еще до того, как нейроны начнут образовывать свои конусы роста. Это следует из того, что филоподии конуса роста МР1 специфически связываются с телом нейрона рСС, но не аСС, хотя и находятся в одинаковом положении относительно обеих клеток. Различия между этими сестринскими клетками становятся яснее с момента появления у них конусов роста. Конус роста нейрона рСС продвигается вперед так, что оказывается в одном пучке с аксонами МР 1 и dMP2. Конус роста нейрона аСС также направляется вперед, но вместо того, чтобы расти дальше, остается в таком начальном положении на 10—15 ч— до тех пор, пока растущие аксоны U1 и U2 не пересекут район, доступный для его филоподий. Тогда конус роста аСС поворачивается по направлению к этим аксонам, соединяется с ними и следует с ними назад. Для проверки гипотезы меченых путей С. дю Лак убивал при помощи лазера нейроны U1 и U2. В их отсутствие только что появившийся конус роста аСС так и оставался в начальном положении и ничуть не «желал» расти вдоль других нейронных поверхностей, которых достигали его филоподин. Сходно, когда убивали нейроны МР 1 и dMP2, конус роста нейрона рСС не проявлял никакого сродства к другим аксонам.

 

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